Reconstitution
Peptide Reconstitution
Research Use Only · Not for Human Consumption
Reconstitution is the process of restoring a lyophilized peptide to its liquid form by adding an appropriate sterile solvent. The standard protocol involves slowly adding bacteriostatic water along the side of the vial (to avoid foaming), then gently swirling until the powder fully dissolves. Reconstituted peptide solutions are stored at 4°C and typically used within 28 days. Shaking is avoided as it can denature the peptide.
Key Facts
- Standard solvent
- Bacteriostatic water
- Procedure
- Add solvent slowly, swirl gently
- Post-reconstitution storage
- 4°C, use within 28 days
- Avoid
- Vigorous shaking (causes denaturation)
Research Applications
- Preparing lyophilized peptides for research use
- Diluting concentrated peptide solutions
- Research protocol preparation
Frequently Asked Questions
Related Terms
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